Temperature
pH
Substrate Concentration
Parameters
Enzyme preset
Human enzyme (37°C)
Thermophile (75°C)
Psychrophile (10°C)
Optimum temp
37°C
Key values
Optimum
37°C
Denaturation starts
~45°C
Below optimum:
Slow — low kinetic energy
At optimum:
Maximum activity
Above optimum:
Denaturation — active site permanently lost
Enzyme preset
Amylase (pH 7)
Pepsin (pH 2)
Trypsin (pH 8)
Optimum pH
7.0
Key values
Optimum pH
7.0
Active range
5–9
Optimal pH
maintains correct charge on active site amino acids.
Extreme pH
disrupts ionic bonds → denaturation
Enzyme concentration
1.0×
Key values
Vmax
—
At saturation
All sites occupied
Low [S]:
Rate ∝ [S] — active sites available
High [S]:
Rate levels off — all sites occupied (Vmax)
Enzyme Activity vs. Temperature
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Interactive Graph