Biology> Year 11> Module 3> Lesson 10

Variation and Allele Frequency

Natural selection cannot act unless variation already exists. This lesson explains where variation comes from, how allele frequency describes a population genetically, and why selection pressure changes populations over generations rather than transforming individual organisms on demand.

IQ3 ~50 min Lesson 10 of 18 5 MC + 3 short answer
📊

Choose how you work — type your answers below or write in your book.

Feedback Loop Diagram A negative feedback loop showing stimulus, receptor, control centre, effector and response. STIMULUS RECEPTOR CONTROL CENTRE EFFECTOR RESPONSE Negative feedback restores homeostasis detects sends signal sends signal carries out

Use digital mode if you want to work through the allele-frequency reasoning and the quantitative drug-resistance example directly on-screen. Switch to book mode if you want to sketch gene-pool changes and calculation steps by hand before checking your answers here.

Printable worksheet

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Use the PDF for classwork, homework or revision. It includes key ideas, activities, questions, an extend task and success-criteria proof.

Think First

Lock in your first instinct before we formalise the mechanism.

1. If 4% of a population carries a resistant allele before treatment, can that percentage change after a strong selection pressure is applied? Why?

2. If one organism survives a harsh environment, has that organism evolved, or has something else changed?

Write your initial answer now. We will revisit it after the allele-frequency and drift comparisons.

Write your starting answer in your book, then return later to compare it with your final explanation.

Write this in your book, then revisit it later.
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📚 Know

  • Key facts and definitions for Variation and Allele Frequency
  • Relevant terminology and conventions

🔗 Understand

  • The concepts and principles underlying Variation and Allele Frequency
  • How to explain the reasoning behind key ideas

✅ Can Do

  • Apply concepts from Variation and Allele Frequency to exam-style questions
  • Justify answers using appropriate biological reasoning
Key Terms
What allele frequencyand how it is expressed
Why mutationthe ultimate source of new alleles
Bacterialiving cells; viruses are non-living particles that require host cells to reproduce
Understanding how systems interactessential for HSC success
Mutationthe ultimate source of all new alleles because it changes the DNA sequence itself
Most mutationsneutral, some are harmful, and only occasionally does a mutation become advantageous in a specific environment

Know

  • The main sources of variation in a population.
  • What allele frequency means and how it is expressed.
  • The difference between natural selection and genetic drift.

Understand

  • Why mutation is the ultimate source of new alleles.
  • How selection pressure changes allele frequency over generations.
  • Why populations evolve but individuals do not.

Can Do

  • Explain how variation enters and moves through populations.
  • Interpret a simple quantitative allele-frequency scenario.
  • Distinguish non-random selection from random drift.
Key Terms — scan these before reading
Definition relevant to Variation and Allele Frequency.
Definition relevant to Variation and Allele Frequency.
Definition relevant to Variation and Allele Frequency.
Definition relevant to Variation and Allele Frequency.
Definition relevant to Variation and Allele Frequency.
Definition relevant to Variation and Allele Frequency.

Misconceptions to Fix

Wrong: Bacteria and viruses are the same thing.

Right: Bacteria are living cells; viruses are non-living particles that require host cells to reproduce.

Core Content

Key Point

Connect this concept to the broader biology framework. Understanding how systems interact is essential for HSC success.

01

Where Variation Comes From

Mutation, recombination and gene flow as the raw material of evolution

Natural selection cannot create variation out of nothing. It can only act on differences that already exist in a population.

Mutation is the ultimate source of all new alleles because it changes the DNA sequence itself. Most mutations are neutral, some are harmful, and only occasionally does a mutation become advantageous in a specific environment. Genetic recombination does not create brand-new alleles, but it shuffles existing alleles into new combinations during meiosis and sexual reproduction. Gene flow adds another source of population-level change because migrants bring alleles in and take alleles out.

MutationCreates new alleles by changing DNA.
RecombinationRearranges existing alleles into new combinations.
Gene FlowMoves alleles between populations through migration.
Source of VariationWhat It DoesWhy It Matters
MutationAlters DNA sequenceUltimate source of new alleles
Genetic recombinationShuffles alleles during meiosis and sexual reproductionCreates new genotype combinations
Gene flowMoves alleles between populationsCan increase or reduce differences between populations
Exam tip: if you are asked for the ultimate source of variation, the safest answer is mutation. Recombination shuffles existing variation; it does not invent new alleles by itself.
02

Allele Frequency and Selection Pressure

How population genetics tracks evolutionary change

Allele frequency is the proportion of a particular allele in the gene pool of a population, often written as a decimal or percentage.

This is important because evolution at the population level can be described as a change in allele frequency over generations. If allele A improves survival or reproduction under a particular selection pressure, individuals carrying A leave more offspring. As a result, A becomes more common in the next generation. That is population evolution in measurable form.

Allele frequency idea: if 4 out of 100 alleles in the population are allele A frequency of A = 0.04 = 4% If selection favours A over generations, frequency of A increases in the gene pool.
Selection Pressure Changes Allele Frequency Before selection After selection Selection pressure Blue circles represent a favoured allele becoming more common in the gene pool over generations.
Natural selection can be visualised as a shift in allele frequency from one generation to the next.
Common misconception: a single organism exposed to selection pressure evolves during its lifetime. The more accurate statement is that differential survival and reproduction change allele frequencies across generations in the population.
03

Worked Example and Selection vs Genetic Drift

Non-random change versus random change

Suppose 4% of a population carries a drug-resistant allele before antibiotic treatment, and 90% of susceptible individuals die before reproducing. The important conclusion is not the exact arithmetic detail first. It is that the resistant allele becomes much more common in the next generation because selection favoured it.

That makes this an example of natural selection rather than genetic drift. Natural selection is non-random with respect to fitness because the environment consistently favours certain variants. Genetic drift is different. Drift changes allele frequencies by chance, especially in small populations, without a specific adaptive reason.

Worked selection idea: Starting resistant allele frequency = 4% Strong selection kills most susceptible individuals Resistant carriers leave proportionally more offspring New resistant allele frequency in the next generation rises sharply Key takeaway: selection changes allele frequency for a reason drift changes allele frequency by chance
ProcessCause of ChangePattern
Natural selectionSelection pressure favours some variants over othersNon-random change in allele frequency
Genetic driftChance events, especially in small populationsRandom change in allele frequency
Assessment angle: if a question gives you a survival advantage linked to an environmental pressure, you are usually looking at natural selection. If the question focuses on random survival in a small population, you are more likely dealing with drift.

Sources of Variation

  • Mutation creates new alleles.
  • Recombination shuffles alleles and gene flow moves them between populations.

Allele Frequency

  • Allele frequency is the proportion of an allele in the population gene pool.
  • Population evolution can be described as a change in allele frequency over generations.

Selection Pressure

  • If an allele gives an advantage under a pressure, its frequency tends to increase.
  • Populations evolve; individuals do not rewrite their DNA in response to need.

Selection vs Drift

  • Natural selection is non-random and linked to fitness.
  • Genetic drift is random and strongest in small populations.

Activities

ApplyBand 3-4
Activity 01

Track the Advantage

Pattern B - Apply mechanism

A beetle population contains a rare dark-colour allele. A forest fire darkens the tree bark, and birds now spot pale beetles more easily. Explain how the frequency of the dark-colour allele could change over several generations.

Link the environmental change to differential survival and then to allele frequency.

Sketch the before-and-after population in your book first, then summarise the explanation here.

Map the change in your book, then write the explanation here.
EvaluateBand 4-5
Activity 02

Selection or Drift?

Pattern B - Distinguish and justify

Two small island populations lose many individuals in a storm at random. In a separate case, a pesticide kills insects without a resistance allele. Decide which case is best explained by drift and which by natural selection, and justify each decision.

Focus on whether the change is random or linked to a fitness advantage.

Make a two-case comparison in your book, then record your final judgement here.

Write the comparison in your book, then condense it here.

Revisit Your Thinking

Once you think in allele frequencies rather than just visible traits, evolution becomes much easier to explain. Selection pressure does not magically create the useful allele. It changes how common that allele becomes in the population over time.

If your original answer focused on one organism changing, the key correction is this: the individual survives or dies, but the population evolves when allele frequencies shift across generations.

Assessment

MC

Check Your Understanding

Answer first, then read the explanation

1. What is the ultimate source of all new alleles in a population?

What is NOT the ultimate source of all new alleles in a population?

2. What does allele frequency describe?

What is NOT does allele frequency describe?

3. Why does selection pressure increase the frequency of some alleles?

4. Which statement correctly distinguishes genetic drift from natural selection?

5. Which statement best captures why populations rather than individuals evolve?

Short Answer - 10 marks

1. Explain how mutation, recombination and gene flow each contribute to variation in a population. (4 marks)

1 mark: mutation | 1 mark: recombination | 1 mark: gene flow | 1 mark: clear comparative explanation

2. Explain how a selection pressure can increase the frequency of a resistant allele in a population over generations. (3 marks)

1 mark: resistant allele advantage | 1 mark: differential reproduction | 1 mark: frequency increase over generations

3. Distinguish between genetic drift and natural selection using one example or scenario for each. (3 marks)

1 mark: drift defined/example | 1 mark: selection defined/example | 1 mark: clear distinction

Answers

SA1: Mutation contributes to variation by creating new alleles through random changes in DNA sequence. Genetic recombination contributes by reshuffling existing alleles during meiosis and sexual reproduction, producing new genotype combinations. Gene flow contributes by moving individuals and their alleles between populations, adding or removing variation from the local gene pool. Together these processes maintain the raw material on which selection can act.

SA2: If a resistant allele gives carriers an advantage under a selection pressure such as antibiotic exposure, those individuals are more likely to survive and reproduce. Because the allele is inherited, more offspring in the next generation carry it. Over multiple generations, the resistant allele becomes more common in the population gene pool.

SA3: Genetic drift is a random change in allele frequency, especially in small populations. For example, a storm might randomly kill many individuals regardless of whether they carry useful alleles. Natural selection is a non-random change in allele frequency caused by differential survival or reproduction under a selection pressure. For example, pesticide resistance becomes more common when insects carrying the resistance allele survive treatment and reproduce. The key difference is that drift is chance-based, while selection is linked to fitness advantage.

AR

Rapid Recall

Say each answer aloud before moving to the next prompt

  1. What is the ultimate source of all new alleles?
  2. How does recombination differ from mutation?
  3. What does allele frequency measure?
  4. Why does selection pressure change allele frequency over time?
  5. Why do populations rather than individuals evolve?
  6. What is the difference between natural selection and genetic drift?