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Biology Year 12 Module 5 Lesson 13

Sources of Genetic Variation - Meiosis, Crossing Over, Fertilisation, Mutation

Variation in offspring is not caused by a single process. Some variation comes from reshuffling existing alleles during meiosis and fertilisation, while mutation creates entirely new alleles.

40 min IQ4 Genetic variation 5 MC · 3 Short Answer Lesson 13 of 19
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Prediction

Think First

Two siblings have the same parents, but they are not genetically identical. A student says, "That must mean one of them got mutations everywhere across the genome. Otherwise siblings should be genetically the same."

Before reading on, explain why that reasoning is weak. How can meiosis and fertilisation create genetic differences between siblings even without new mutations in every gene?

Key Terms
Genetic variationDifferences in genetic makeup within or between individuals of a population.
Crossing overExchange of corresponding chromosome segments between homologous chromosomes during meiosis.
Independent assortmentRandom orientation and separation of homologous chromosomes in meiosis.
Random fertilisationThe chance fusion of one gamete with another, creating many possible genotype combinations.
MutationA change in DNA sequence that can create a new allele.
AlleleAn alternative version of a gene.

Know

  • Major sources of genotype variation in sexually reproducing organisms.
  • That mutation is the source of new alleles.

Understand

  • Why crossing over and independent assortment reshuffle existing alleles.
  • How random fertilisation increases possible genotype combinations.

Apply

  • Distinguish new allele combinations from genuinely new alleles.
  • Explain sibling similarity without identity using meiosis and fertilisation.
1
Core Idea

Variation Comes from Reshuffling and from New Sequence Change

Not all genetic variation is produced in the same way. Some processes rearrange existing alleles, while mutation creates new alleles.

In this lesson, four major sources of variation matter: crossing over, independent assortment, random fertilisation and mutation. The first three mainly create new combinations of already existing alleles. Mutation is different because it can create a genuinely new allele by changing the DNA sequence itself.

Trap
Crossing over does not create new alleles. It reshuffles existing alleles. Mutation creates new alleles.
2
Meiosis

Crossing Over and Independent Assortment Reshuffle Existing Alleles

During meiosis, homologous chromosomes pair and can exchange segments by crossing over. This produces chromosomes containing new combinations of existing maternal and paternal alleles.

Independent assortment also contributes to variation. Homologous chromosome pairs line up randomly, so each gamete receives a different mix of maternal and paternal chromosomes. Together, crossing over and independent assortment mean gametes produced by the same individual are usually not genetically identical.

Crossing Over

  • Occurs between homologous chromosomes
  • Exchanges chromosome segments
  • Creates new allele combinations

Independent Assortment

  • Occurs when homologous pairs separate
  • Randomly distributes whole chromosomes
  • Changes chromosome combinations in gametes
3
Gamete Fusion

Random Fertilisation Increases Genotype Combinations Further

Even after meiosis has produced varied gametes, another source of variation comes from random fertilisation. Any one gamete from one parent may fuse with any one gamete from the other parent, producing many possible zygote genotype combinations.

This is why siblings can be genetically similar but not identical. They inherit alleles from the same parents, but the specific gamete combinations that formed each sibling are different.

Real World
Sibling similarity without identity is a direct consequence of varied gamete formation plus random fertilisation. Identical siblings require a different developmental circumstance, not ordinary fertilisation alone.
4
New Alleles

Mutation Creates New Alleles

Mutation is different from crossing over, independent assortment and fertilisation because it can create a genuinely new allele by changing the DNA sequence. This means mutation is the source of new alleles, not just new combinations of existing ones.

In contrast, meiosis and fertilisation mostly reshuffle alleles that already exist in the population. This distinction matters because many exam errors come from calling every source of variation a "mutation" or saying that crossing over creates new alleles.

Precision
Use this wording carefully: mutation creates new alleles; crossing over, independent assortment and fertilisation create new allele combinations.
5
Model

Concept Map of Variation Sources

Genetic Variation Crossing over new allele combinations Independent assortment Random fertilisation Mutation new alleles reshuffles existing alleles reshuffles chromosomes combines varied gametes changes DNA sequence
Three major sources reshuffle existing variation; mutation introduces new alleles.

Crossing Over

Produces recombinant chromosomes with new allele combinations.

Independent Assortment

Distributes chromosomes into many possible gamete combinations.

Random Fertilisation

Combines different gametes into many possible zygote genotypes.

Mutation

Creates new alleles by altering DNA sequence.

Copy Into Your Books

Core Idea

Genetic variation arises from reshuffling existing alleles and from mutation creating new alleles.

Mechanism / Process

Crossing over, independent assortment and random fertilisation produce new allele combinations, while mutation creates new alleles.

Common Mistake

Do not say crossing over creates new alleles. That is the role of mutation.

Exam Sentence Starter

Genetic variation is produced when meiosis and fertilisation reshuffle existing alleles, while mutation...

Revisit Your Initial Thinking

Look back at what you wrote in the Think First section. What has changed? What did you get right? What surprised you?

Activities

Activity 1 - Sort the Source

For each example, identify whether it is best explained by crossing over, independent assortment, random fertilisation or mutation.

1. A gamete receives a different mix of maternal and paternal chromosomes from another gamete made by the same parent.

2. A new DNA sequence change creates a new allele.

3. Homologous chromosomes exchange corresponding segments.

4. One sperm fuses with one egg out of many possible gamete combinations.

Activity 2 - Sibling Similarity Without Identity

Explain why ordinary siblings can resemble each other genetically but are usually not genetically identical. Use meiosis and fertilisation in your answer.

Multiple Choice

UnderstandBand 3

1. Which process is the source of genuinely new alleles?

A
Crossing over
B
Independent assortment
C
Mutation
D
Random fertilisation
UnderstandBand 3

2. What is the main effect of crossing over?

A
It produces new combinations of existing alleles by exchanging chromosome segments.
B
It always creates a brand new allele by changing base sequence.
C
It keeps all gametes genetically identical.
D
It happens only after fertilisation.
ApplyBand 4

3. Which event best illustrates independent assortment?

A
A change in DNA sequence produces a new allele.
B
Homologous chromosomes exchange segments.
C
A sperm fertilises an egg.
D
Homologous chromosome pairs separate randomly into gametes.
AnalyseBand 4

4. Why are ordinary siblings usually not genetically identical?

A
Because one sibling always has more mutations than the other.
B
Because meiosis produces varied gametes and fertilisation combines them randomly.
C
Because genotype does not affect inheritance.
D
Because all chromosomes in meiosis are copied incorrectly.
EvaluateBand 5

5. Which statement best distinguishes reshuffling from mutation?

A
Crossing over and mutation both create only new alleles.
B
Mutation and fertilisation both simply reshuffle existing alleles.
C
Crossing over, independent assortment and fertilisation reshuffle existing alleles, while mutation can create a new allele.
D
Independent assortment creates new alleles more effectively than mutation.

Short Answer

UnderstandBand 3

6. Distinguish between crossing over and mutation as sources of variation. 3 marks

AnalyseBand 4

7. Explain how meiosis and fertilisation together generate genotype variation in offspring. 4 marks

EvaluateBand 5

8. Evaluate the statement: "Sibling variation can be explained mainly by reshuffling existing alleles, while mutation is important because it introduces new alleles." 5 marks

Rapid Review

Crossing over:
reshuffles existing alleles by exchanging chromosome segments.
Independent assortment:
randomly distributes chromosomes into gametes.
Random fertilisation:
combines different gametes into many possible zygotes.
Mutation:
creates new alleles by changing DNA sequence.

Revisit Your Thinking

You should now be able to reject the idea that sibling differences require mutation everywhere. Most sibling variation is explained by meiosis reshuffling existing alleles and fertilisation combining gametes randomly, while mutation introduces genuinely new alleles.

Answers and Explanations

Activity 1 - Sort the Source

1. Independent assortment.

2. Mutation.

3. Crossing over.

4. Random fertilisation.

Activity 2 - Sibling Similarity Without Identity

Siblings inherit alleles from the same parents, so they are genetically similar. However meiosis produces genetically varied gametes through crossing over and independent assortment, and fertilisation combines these gametes randomly. This means siblings are usually not genetically identical.

Multiple Choice

1. C - Mutation is the source of genuinely new alleles.

2. A - Crossing over creates new combinations of existing alleles.

3. D - Independent assortment is the random separation of homologous chromosome pairs.

4. B - Meiosis plus random fertilisation explains most sibling genetic differences.

5. C - Reshuffling and mutation contribute to variation in different ways.

Short Answer Model Responses

Q6 (3 marks): Crossing over exchanges segments between homologous chromosomes during meiosis and creates new combinations of existing alleles [1]. Mutation is a change in DNA sequence [1]. Mutation can create a new allele, whereas crossing over mainly reshuffles alleles that already exist [1].

Q7 (4 marks): Meiosis generates genotype variation because crossing over creates new allele combinations and independent assortment distributes chromosomes randomly into gametes [1]. This means gametes produced by the same parent are usually genetically different [1]. Fertilisation then adds variation because any one gamete from one parent may fuse with many possible gametes from the other parent [1]. Together, meiosis and fertilisation produce many possible offspring genotypes [1].

Q8 (5 marks): The statement is valid because most variation between ordinary siblings is explained by reshuffling of existing alleles rather than new mutation in every gene [1]. Crossing over and independent assortment during meiosis produce genetically varied gametes [1]. Random fertilisation then combines these gametes in many possible ways [1]. Mutation remains important because it introduces new alleles by changing DNA sequence [1]. Therefore sibling variation is largely explained by reshuffling, while mutation is the source of genuinely new alleles in a population [1].

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