BiologyYear 12Module 7Checkpoint 2

Checkpoint Quiz 2

Covers Lessons 08–13: plant and animal responses to pathogens, innate immunity, adaptive immunity (humoral and cell-mediated), and primary vs secondary immune response.

20 Multiple Choice 3 Short Answer Lessons 08–13
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L08

Plant responses to pathogens — physical, chemical, hypersensitive response, Banksia

L09

Animal physical/chemical responses — inflammation, fever, chemical mediators

L10

Innate immune system — phagocytosis, NK cells, complement, interferons

L11

Antigens, antibodies, clonal selection, humoral immunity, memory B cells

L12

T cells, cell-mediated immunity, MHC classes, T helper coordination

L13

Primary/secondary response, vaccination, Jenner, herd immunity, passive immunity

Multiple Choice — 20 Questions
L08 — Plant Responses
1

The hypersensitive response (HR) in plants is best described as:

A A generalised immune response involving circulating white blood cells that destroy invading pathogens
B The production of phytoalexins throughout the entire plant to prevent systemic infection
C Programmed death of infected and adjacent cells, creating a necrotic zone that biotrophic pathogens cannot exploit
D Rapid closure of stomata across all leaf surfaces to prevent pathogen entry
L08 — Plant Responses
2

Salicylic acid (SA) produced at a localised Phytophthora infection site in a Banksia root travels to uninfected shoots. This represents:

A The hypersensitive response spreading systemically to trigger cell death throughout the plant
B Systemic acquired resistance — activating PR gene expression throughout the plant to prime broad-spectrum defence
C A constitutive defence mechanism that is always present in Banksia tissue
D Phytoalexin transport from the infection site to healthy tissue to directly kill the pathogen
L09 — Animal Responses
3

The swelling observed at a site of bacterial infection is caused primarily by:

A Vasodilation — increased blood flow causing fluid accumulation in blood vessels
B Neutrophils physically enlarging at the infection site as they engulf bacteria
C Increased capillary permeability allowing plasma to leak into surrounding tissue
D Prostaglandins directly stimulating tissue growth around the infection site
L09 — Animal Responses
4

Ibuprofen reduces fever and pain by inhibiting the enzyme COX (cyclooxygenase). This works because COX is required for the synthesis of:

A Histamine — the primary mediator of vasodilation and capillary permeability
B Prostaglandins — which sensitise pain receptors and act as pyrogens to raise body temperature
C Complement proteins — which opsonise bacteria and form the membrane attack complex
D Cytokines — which recruit neutrophils to the infection site
L09 — Animal Responses
5

Interferons are released by virus-infected cells. Their primary function is to:

A Directly destroy virus particles circulating in the bloodstream
B Trigger mast cells to release histamine, initiating the inflammatory response
C Activate complement proteins to coat viral particles for phagocytosis
D Signal neighbouring uninfected cells to produce antiviral proteins, limiting viral spread before adaptive immunity activates
L10 — Innate Immune System
6

Natural killer (NK) cells detect virus-infected host cells by:

A Binding to viral PAMPs displayed on the infected cell surface using pattern recognition receptors
B Recognising viral peptides presented on MHC class I — the same mechanism as cytotoxic T cells
C Detecting the absence or reduction of MHC class I — a "missing self" signal that indicates viral infection or malignancy
D Receiving antibody signals from B cells that have recognised the infected cell
L10 — Innate Immune System
7

During phagocytosis, the phagolysosome is formed when:

A The phagocyte extends pseudopods around the pathogen, beginning the engulfment process
B A lysosome containing digestive enzymes fuses with the phagosome enclosing the engulfed pathogen
C Antigen fragments from the digested pathogen are loaded onto MHC class II molecules
D Complement proteins coat the pathogen surface, facilitating adherence to the phagocyte
L10 — Innate Immune System
8

Dendritic cells are described as bridging innate and adaptive immunity because they:

A Produce both antibodies and phagocytose pathogens simultaneously
B Are found in both blood and tissues and therefore act in both immune compartments
C Release interferons that directly activate B cells without antigen presentation
D Engulf pathogens as part of the innate response, then migrate to lymph nodes to present antigens to T cells, activating the adaptive response
L11 — Antigens and Antibodies
9

The variable region of an antibody molecule determines:

A The specific antigen-binding site — its unique shape determines which epitope the antibody can bind
B The antibody class (IgG, IgM etc.) and the effector functions available
C The ability of the antibody to bind to Fc receptors on phagocytes during opsonisation
D Whether the antibody triggers complement activation or direct neutralisation
L11 — Antigens and Antibodies
10

Clonal selection refers to the process by which:

A Memory B cells select the most effective antibody from a previous infection to respond to a new pathogen
B The bone marrow produces large numbers of identical naive B cells for deployment during infection
C The specific B cell whose receptor matches the antigen is identified and activated while all others remain inactive
D Cytotoxic T cells select which infected cells to destroy based on the concentration of antigen displayed
L11 — Antigens and Antibodies
11

An antibody binds to a bacterial surface protein, making the bacterium significantly easier for neutrophils to engulf. This mechanism is called:

A Neutralisation — the antibody blocks the bacterium from binding to host cell receptors
B Opsonisation — the antibody coating allows phagocytes to bind the Fc region and engulf the bacterium more efficiently
C Agglutination — the antibody clumps multiple bacteria together, preventing their spread
D Complement activation — the antibody triggers the membrane attack complex to puncture the bacterium
L12 — T Cells and Cell-Mediated Immunity
12

MHC class I molecules differ from MHC class II molecules in that MHC class I:

A Is found only on antigen-presenting cells such as dendritic cells and macrophages
B Is found on all nucleated body cells and presents intracellular peptides to cytotoxic T cells (CD8+)
C Presents extracellular antigen fragments to T helper cells (CD4+) for immune coordination
D Is the molecule that NK cells detect — its presence signals that a cell is infected
L12 — T Cells and Cell-Mediated Immunity
13

When a cytotoxic T cell kills a virus-infected host cell, the mechanism involves:

A Phagocytosis — the cytotoxic T cell engulfs the infected cell and digests it with lysosomal enzymes
B Complement activation — the T cell releases complement proteins that form a membrane attack complex on the infected cell
C Release of perforin (pore-forming protein) and granzymes (proteases) that trigger apoptosis in the target cell
D Release of antibodies that neutralise virus particles inside the infected cell before they are released
L12 — T Cells and Cell-Mediated Immunity
14

HIV progressively destroys CD4+ T helper cells. The most significant immunological consequence is that:

A NK cells can no longer detect virus-infected cells because T helper cells provide the "missing self" detection signal
B The innate immune system shuts down entirely because T helper cells are required for all innate responses
C Phagocytes lose their ability to perform phagocytosis because T helper cell cytokines are required for pseudopod formation
D Both humoral and cell-mediated adaptive immunity are severely impaired — B cells and cytotoxic T cells both require T helper signals for full activation
L13 — Primary and Secondary Response
15

The secondary immune response produces antibody levels 10–100 times higher than the primary response primarily because:

A The pathogen is recognised as weaker on second exposure, so the immune system responds more vigorously to compensate
B Memory B cells formed during the primary response rapidly differentiate into plasma cells without the slow naive clonal selection process
C Residual antibodies from the primary response directly stimulate further antibody production on re-exposure
D The secondary response uses IgM rather than IgG, which is a more potent antibody class
L13 — Vaccination
16

Edward Jenner's cowpox vaccination protected against smallpox because:

A Cowpox permanently occupied the receptor sites that smallpox would use to infect host cells
B Cowpox vaccination stimulated innate immunity, which remained permanently elevated against all viral infections
C Cowpox and smallpox share antigenic similarities — memory B and T cells raised against cowpox antigens cross-reacted with smallpox antigens, enabling a rapid secondary response
D Cowpox directly killed smallpox virus particles in the bloodstream through competitive exclusion
L13 — Vaccination
17

Passive immunity differs from active immunity in that passive immunity:

A Provides immediate but temporary protection via pre-formed antibodies, without generating immunological memory
B Requires T cells rather than B cells and therefore does not involve antibody production
C Is only effective against viruses — active immunity is required for bacterial infections
D Produces stronger immunity than active immunisation because the antibodies are already fully mature
Integration — L08 + L12
18

The hypersensitive response (HR) in plants and cytotoxic T cell killing in animals are both strategies for limiting pathogen spread. Which statement correctly compares these two mechanisms?

A Both mechanisms involve programmed cell death of the host cell — making them functionally identical despite being in different organisms
B Both involve deliberate host cell death to contain the pathogen, but HR is triggered by pathogen PAMPs (non-specific) while CTL killing requires specific antigen recognition on MHC class I (highly specific)
C The HR produces memory — the plant will respond faster on second exposure — while CTL killing does not produce memory cells
D Both are part of the adaptive immune system because they require specific antigen recognition before being activated
Integration — L10 + L11
19

A patient with a genetic deficiency that prevents complement protein production would most likely experience difficulty with which combination of immune functions?

A Opsonisation of bacteria, membrane attack complex formation, and attracting phagocytes to infection sites
B Antibody production, clonal selection of B cells, and memory B cell formation
C NK cell activation, interferon production, and fever response
D T helper cell activation, cytotoxic T cell killing, and MHC class I expression
Integration — L09 + L13
20

A person receives their first influenza vaccine. Two weeks later they experience mild fatigue and a low-grade fever. A year later, after receiving their annual influenza booster, they feel nothing. Which explanation best accounts for this difference?

A The second vaccine contained fewer antigens, so less immune activation was needed
B The mild symptoms after the first vaccine indicate an allergic reaction that resolved; the second vaccine did not trigger the same reaction
C The first vaccine triggered a primary response — cytokine release caused mild systemic symptoms (fever, fatigue). The booster triggered a faster secondary response via memory cells, clearing the antigen before sufficient cytokine levels built up to cause noticeable symptoms
D After the first vaccine, innate immunity became permanently activated against influenza antigens, preventing any further inflammatory response

Multiple Choice Result

Short Answer — 3 Questions
SA1

Compare the innate and adaptive immune systems across four features: speed, specificity, memory, and the key cells involved. Explain why both systems are necessary for effective defence against pathogens. (4 marks)

1 mark per correctly compared feature (speed, specificity, memory, key cells) | 1 mark: explanation of why both are necessary (innate provides immediate containment; adaptive provides targeted elimination and memory)

Speed: The innate immune system responds within minutes to hours of pathogen detection — always ready, pre-formed. The adaptive immune system requires 7–14 days to reach peak effectiveness as naive B and T cells must undergo clonal selection, clonal expansion, and differentiation.

Specificity: The innate system is non-specific — it uses pattern recognition receptors (PRRs) to detect broad molecular patterns (PAMPs) shared by many pathogens (e.g. LPS, viral RNA). The adaptive system is highly specific — each B or T cell has a unique receptor for one specific antigen.

Memory: Classical innate immunity has no memory — the same response occurs every time. The adaptive system generates immunological memory (memory B and T cells) that enables a faster, stronger secondary response on re-exposure.

Key cells: Innate: neutrophils, macrophages, dendritic cells, NK cells, mast cells. Adaptive: B lymphocytes (→ plasma cells and memory B cells), T helper cells (CD4+), cytotoxic T cells (CD8+).

Why both are necessary: The innate system is essential for immediate containment — without it, pathogens would replicate unchecked during the days it takes the adaptive system to respond. The adaptive system is essential for targeted pathogen elimination, clearing intracellular infections (via CTLs) that the innate system cannot resolve, and forming lasting memory. The innate system also activates and shapes the adaptive response — dendritic cells bridge the two systems.

SA2

Describe the sequence of events from antigen entry to antibody production in humoral immunity. Then explain the role of T helper cells in this process and what would happen if T helper cells were absent. (4 marks)

1 mark: antigen presentation by dendritic cell on MHC II | 1 mark: clonal selection of matching B cell + T helper co-stimulation | 1 mark: clonal expansion → plasma cells + memory B cells | 1 mark: consequence of T helper absence — inadequate B cell activation, no class switching, impaired memory

When an antigen enters the body, dendritic cells (and macrophages) engulf it, process it, and present antigen fragments on MHC class II molecules. These antigen-presenting cells migrate to lymph nodes where naive B cells circulate. Through clonal selection, the rare B cell whose B cell receptor (BCR) matches the specific antigen binds it. However, this alone is insufficient for full activation — the B cell also requires a co-stimulatory signal from a T helper cell (CD4+) that has independently recognised the same antigen on MHC class II. This dual signal prevents accidental B cell activation against self-antigens. Once activated, the B cell undergoes clonal expansion — rapidly dividing to produce a large clone. These differentiate into plasma cells (short-lived antibody factories secreting thousands of specific antibodies per second) and memory B cells (long-lived, persist for years).

If T helper cells were absent (as in late-stage HIV infection), B cells cannot receive the co-stimulatory signal required for full activation and antibody class switching. They may produce some IgM (T-independent antigens can trigger limited responses) but cannot generate high-affinity IgG or form effective memory. Both arms of adaptive immunity are compromised simultaneously.

SA3

A previously unvaccinated adult is exposed to tetanus bacteria through a deep wound. They are treated with tetanus immunoglobulin (pre-formed antibodies) and then given their first tetanus toxoid vaccine. Ten years later they receive a tetanus booster. Using your knowledge of passive and active immunity, primary and secondary immune responses, and memory cells, explain: (i) why the immunoglobulin was given immediately; (ii) what the vaccine did; and (iii) why a booster was needed ten years later. (4 marks)

1 mark: immunoglobulin = passive immunity — immediate protection while adaptive response develops | 1 mark: vaccine = active immunisation — primary response → memory cells | 1 mark: booster = secondary response via memory cells — faster, higher | 1 mark: booster needed because memory cell numbers and antibody levels decline over time without re-exposure

(i) Immunoglobulin — immediate passive immunity: Tetanus toxin acts rapidly and can cause life-threatening illness before the adaptive immune system has time to respond. The adult has no prior immunity (no memory cells, no circulating antibodies). Tetanus immunoglobulin provides passive immunity — pre-formed antibodies that immediately neutralise tetanus toxin. This is temporary (the antibodies are gradually catabolised over weeks) but provides protection during the critical period. No memory cells are formed — passive immunity does not trigger clonal selection.

(ii) Vaccine — active immunisation, primary response: The tetanus toxoid vaccine introduces inactivated tetanus toxin (toxoid) — an antigen that cannot cause disease. The immune system mounts a primary response: dendritic cells present tetanus antigens, the matching B cell clone undergoes clonal selection (with T helper co-stimulation), and clonal expansion produces plasma cells (generating tetanus-specific antibodies) and memory B and T cells. These memory cells persist long-term. The vaccine takes 1–2 weeks to provide effective active immunity — which is why the immunoglobulin was needed in the interim.

(iii) Booster — secondary response, reinforce fading memory: Memory B and T cell populations are not permanent — without re-exposure or booster doses, their numbers decline gradually over years, and antibody levels fall below the protective threshold. Ten years after the primary vaccine, protection may have waned. The booster dose re-exposes the immune system to tetanus toxoid, triggering a secondary response: memory B cells rapidly activate and differentiate into plasma cells within 1–3 days, producing high-affinity IgG at much higher levels than the original primary response. The booster both restores protective antibody levels immediately and expands the memory cell population — resetting the clock on protection for another decade.

← Lesson 13: Primary and Secondary Response Lesson 14: Vaccination — Active and Passive Immunity →