Chromatography — Chromatography Explorer

Explore separation techniques and calculate Rf values

Principle: Separation based on differential partitioning between the stationary phase (paper fibres + bound water) and the mobile phase (solvent moving up the paper). Components with stronger affinity for the stationary phase travel slower.

Chromatogram

Rf Values (after running)

Click "Run Chromatogram" to see separation and calculate Rf values.

Rf = distance spot travelled / distance solvent travelled
0 ≤ Rf ≤ 1 (always)

Compare Mixtures to Identify Components

Select a mixture to analyse. Match spots by colour and Rf value to identify components.